Rat lingual lipase. Characteristics of enzyme activity.
نویسندگان
چکیده
منابع مشابه
Rat Lingual Lipase
The lingual serous glands of rat tongue secrete a potent lipase that hydrolyzes long chain triglycerides. The characteristics of this lipase were studied in preparations of lingual glands from Sprague-Dawley rats. Lipase activity was measured by the release of free fatty acids from emulsified tri[3H]oleate. Lipase activity in the 100,000 x g supernatant of lingual serous gland homogenate was ex...
متن کاملLingual lipase in cystic fibrosis. Quantitation of enzyme activity in the upper small intestine of patients with exocrine pancreatic insufficiency.
We have measured the level of lingual lipase activity in gastric and duodenal aspirates of five patients with cystic fibrosis (CF) and pancreatic insufficiency. Lingual lipase activity (measured in vitro by the hydrolysis of long-chain triglyceride, tri-[3H]olein, at pH 4.2 and expressed in nanomoles FFA released per milliliter aspirate per minute) and pH in gastric and duodenal aspirates were ...
متن کاملLipoprotein Lipase Activity of Rat Cardiac Muscle
1. Subcellular fractions, characterized by using morphological, compositional and enzymic markers, were prepared from rat heart tissue and cells isolated from the hearts of fed and 24h-starved rats. 2. The lipoprotein lipase activity of fractions from whole tissue and isolated cells was determined in either fresh fractions or in acetone/diethyl ether powders of the fractions. 3. Lipoprotein lip...
متن کاملThe reciprocal regulation of lipoprotein lipase activity and hormone-sensitive lipase activity in rat adipocytes.
The regulation of lipoprotein lipase activity was studied in rat adipocytes. Incubation of fat cells for 60 mm in buffer without glucose and insulin resulted in a 50% decrease in lipoprotein lipase activity. This decrease was prevented by glucose and insulin. Inhibition of fat cell protein synthesis by cycloheximide abolished the effect of glucose and insulin and caused a rapid decay of lipopro...
متن کاملEnzyme-linked immunosorbent assay for rat hepatic triglyceride lipase.
A noncompetitive enzyme-linked immunosorbent assay to measure rat hepatic triglyceride lipase (H-TGL) was developed. Antibodies to rat H-TGL were purified from goat antisera by immunoadsorption on an H-TGL-Sepharose 4B column. Routinely, Immulon 2 Removawell strips were coated with the purified antibody overnight at 4 degrees C. After blocking the wells with bovine serum albumin (BSA) for 2 hr ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1979
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)86437-2